influence of added vitamin c and vitamin e on frozen-thawed bovine sperm cryopreserved in citrate and tris-based extenders

نویسندگان

reza asadpour

razi jafari

hossein tayefi - nasrabad

چکیده

the objective of this study was to evaluate quality of frozen-thawed bull semen processed with extenders containing vitamin c and e as antioxidants. pooled semen's were collected from 5 bulls and diluted to a concentration of 30 × 106 sperm/ml with citrate –egg yolk (cey) or tris – egg yolk (tey) extenders. the diluted semen was divided to 5 aliquots including control and 4 experimental groups. each aliquot was further diluted with an equal volume of cey or a tris (hydroxymethyl) aminomethane (tris) - based extender without (control) or containing vitamin c 1mm or 2mm and vitamin e 0.1mm or 0.2mm, and routine semen evaluations like sperm motility, viability and measurement of lipid peroxidation (lpo) were conducted. significant reductions of lpo were achieved by addition of 1mm vitamin c and 0.1 mm vitamin e to cey extender (p < 0.05). the highest sperm viability was achieved by addition of 0.1mm vitamin e to cey extender. supplementing cey extender with 2mm vitamin c and 0.1mm vitamin e improved the sperm motility compared with the control group. on the basis of the present results it is concluded that vitamin c and e are very efficient antioxidants in cey extender.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Influence of Added Vitamin C and Vitamin E on Frozen-Thawed Bovine Sperm Cryopreserved in Citrate and Tris-Based Extenders

The objective of this study was to evaluate quality of frozen-thawed bull semen processed with extenders containing vitamin C and E as antioxidants. Pooled semen's were collected from 5 bulls and diluted to a concentration of 30 × 106 sperm/mL with citrate –egg yolk (CEY) or Tris – egg yolk (TEY) extenders. The diluted semen was divided to 5 aliquots including control and 4 experimental groups....

متن کامل

Original Article Influence of Added Vitamin C and Vitamin E on Frozen-Thawed Bovine Sperm Cryopreserved in Citrate and Tris-Based Extenders

The objective of this study was to evaluate quality of frozen-thawed bull semen processed with extenders containing vitamin C and E as antioxidants. Pooled semen's were collected from 5 bulls and diluted to a concentration of 30 × 10 6 sperm/mL with citrate –egg yolk (CEY) or Tris – egg yolk (TEY) extenders. The diluted semen was divided to 5 aliquots including control and 4 experimental groups...

متن کامل

O-7: Detrimental Effects of Dietary Fish Oil without Vitamin E Supplementation on Cryopreserved Sperm of Iranian Mehraban Rams

Background Although several studies confirmed positive effects of fish oil on semen quality, the antioxidant status in omega-3 supplemented diets of several studies is a fuzzy point. The aim of this study was to investigate the effect of dietary fish oil and (or) vitamin E supplementation on cryopreserved sperm in Mehraban rams. MaterialsAndMethods Sixteen fertile rams were randomly allotted to...

متن کامل

Antioxidative capacity of vitamin E, vitamin C and their combination in cryopreserved Bhadavari bull semen

Aim: The aim was to determine the antioxidative capacity of vitamin E, vitamin C and their combination (vitamin E+C) on standard semen parameters i.e., mortality, percent live sperms, percent abnormal sperms and acrosomal integrity in cryopreserved Bhadawari bull semen. Materials and Methods: Ejaculates collected from four Bhadawari bulls were evaluated and later pooled at 37°C. Pooled semen sa...

متن کامل

Effects of Cigarette Smoke, Alcohol, Vitamin E and Vitamin C on Oral Mucosa and Salivary Peroxides Enzyme in Rats

Introduction: According to studies, high intake of fruits and vegetables are associated with reduced cancer incidence and mortality. The high levels of antioxidants in fruits and vegetables are believed to contribute in cancer prevention, possibly by inhibiting oxidative stress. This study aimed to investigate the effects of cigarette smoke, alcohol and vitamin E and vitamin C on oral mucosa an...

متن کامل

The Effect of Vitamin E and Selenium on Sperm Chromatin Quality in Couples with Recurrent Miscarriage

Background and Aims: Due to the paucity of studies, association between the morphology and function of sperm and recurrent miscarriage (RM) is not yet completely known. Increased reactive oxygen species and decreased antioxidant levels in men have been shown to be associated with RM. Recently it has been accepted that antioxidant therapy can approve sperm parameters. The goal of this study was ...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
veterinary research forum

ناشر: faculty of veterinary medicine, urmia university

ISSN 2008-8140

دوره 2

شماره 1 2012

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023